Stainless Steel Bioreactor

CBR500 series pilot bioreactor is an integrated control system available both in microbial and cell culture. As stainless-steel vessels, it standards with the capacities from 10L to 500L. Customized bioreactors are available for engineering solutions up to 20ton. As part of the comprehensive bioreactor portfolio, those stainless steel fermenters can be easily integrated as a component of multi-stage systems.

Make Pilot Production

CBR500 Series

Features

01

Culture Vessel

Available culture vessels from 10L, 20L, 30L, 50L, 100L and 200L total volume, with a total volume aspect ratio of (H:D) 2:1 or 3:1, up to 75% working volume.

Contact us for larger volume

03

CIP Station

Optional movable CIP cleaning station with its own control system, allowing the operator to selectively, effectively, repeatedly clean the system.

02

Sterilization in Place [SIP]

Easy to operate, in-situ sterilization includes fermenter, intake filter and pipeline, exhaust pipeline; Sampling valve, inoculation port and discharge valve can be sterilized manually or automatically.

04

Data Storage and Protection

Data protection: Overlimit alarm function; The real-time curve can be restored as it is when power is cut off and restarted.

Gassing Strategies

Application-appropriate spargers are available for microbiology and cell culture. The system selects corresponding gassing strategies for different sizes and types of fermenters. And the gas flow rate can be adjusted manually by a precision rotameter or automatically by an optional MFC.

CELL CULTURE

Additive Flow

Simultaneous gassing strategy of overlay and sparger: air goes into overlay through a flow meter and a solenoid valve. Solenoid valves select air, O2, N2 and CO2 to flow to the sparger. An additional airflow path can be added to the distributor. Mass flowmeter is optional for each gas inlet.

Model	Total Volume	Working Volume
BR500-C1-500L	≈500L	/
BR500-C1-300L	≈300L	/
BR500-C1-200L	198L	148.5
BR500-C1-100L	119L	89L
BR500-C1-50L	58L	43.5L
BR500-C1-30L	34L	25.5L
BR500-C1-20L	21L	15.8L
BR500-C1-15L	16L	12L
BR500-C1-10L	11.5L	8.6L

Stirrer shaft with top single mechanical seal
Defoaming blade * 1
Marine blade * 1
4-baffles [removable]
Sterilizing filter with stainless steel housing
Aeration tube with ring sparger
Pressure gauge [0~0.4Mpa]
Pressure sensor
Top light, side viewing mirror
CIP spray ball * 2

Exhaust condenser
Side resterilizable sampling valve
Resterilizable bottom harvest valve
Single channel Sacova valve, no needle addition
Three-way Sacova valve, no needle addition
Integrated skid design
PH electrode, cable [polarography]
DO electrode, cable
Temperature sensor PT 100
Foam electrode, cable

Top magnetic seal
Stirrer shaft with double mechanical seal
Movable CIP station with independent controller
In-line sterilizable feed valve group
Vessel weight measurement [Weighing module or differential pressure levelmeter]
Auto vessel pressure control
Lid lifting device [electric]
Mass flowmeter

Dual PH, DO and defoam sensors
DO sensor, cable [optical]
Extra peristaltic pump
Turbidity detection
Redox detection
Exhaust detection
Liquid level monitoring
Qualification Documents

MICROBIAL RESEARCH

O₂-Enrichment

Utilizing two flow paths for air and O2 flow, the flow meter intuitively indicates and allows manual adjustment of the sparger flow rate. O2 is pulsed through a solenoid valve and flows only as needed to maintain the Dissolved Oxygen (DO) set point. No air is provided at this time. A mass flow controller can be integrated to measure and control the total gas flow range by manual adjustment or automatically combined with a DO controller.

Temperature, DO [cascade control], agitation speed
Foam sensor, foam alarm
Tank pressure display [pressure sensor]
Auto sterilization in place [automatic sequence]
Control PH by adding acid/alkali
Rotameter, gassing strategy
Solenoid valve [for O2]
Low noise motor, maintenance-free
Variable speed peristaltic pump
Control: Industrial PC
Display & Operation: Touch Panel 15" | Touch Screen

Model	Total Volume	Working Volume
BR500-M1-300L	≈300L	/
BR500-M1-500L	≈500L	/
BR500-M1-200L	198L	148.5
BR500-M1-100L	119L	89L
BR500-M1-50L	58L	43.5L
BR500-M1-30L	34L	25.5L
BR500-M1-10L	11.5L	8.6L
BR500-M1-15L	16L	12L
BR500-M1-20L	21L	15.8L

Stirrer shaft with top single mechanical seal
4-blade pitched impeller [vessel>10L]
1 x Defoaming blade; 2 x Rushton 6-blade impeller
4-baffles [removable]
Sterilizing filter with stainless steel housing
Aeration tube with ring sparger
Pressure gauge [0~0.4Mpa]
Pressure sensor
Top light, side viewing mirror
CIP spray ball * 2

Exhaust condenser
Side resterilizable sampling valve
Resterilizable bottom harvest valve
Single channel Sacova valve, no needle addition
Three-way Sacova valve, no needle addition
Integrated skid design
PH electrode, cable [polarography]
DO electrode, cable
Temperature sensor Pt 100
Foam electrode, cable

Top magnetic seal
Stirrer shaft with double mechanical seal
Movable CIP station with independent controller
In-line sterilizable feed valve group
Vessel weight measurement [Weighing module or differential pressure levelmeter]
Auto vessel pressure control
Lid lifting device [electric]
Mass flowmeter

Dual PH, DO and defoam sensors
DO sensor, cable [optical]
Extra peristaltic pump
Turbidity detection
Redox detection
Exhaust detection
Liquid level monitoring
Qualification Documents

CBR500 PROJECTS

To play, press and hold the enter key. To stop, release the enter key.

AIRLIFT BIOREACTOR

Gas Cultured

Airlift fermenter & bioreactor is a gas-liquid bioreactor based on the principle of ventilation pipes, without any mechanical stirring. Compressed air is used for aeration and agitation. The turbulence caused by the gas/fluid flow ensures adequate mixing of the liquid. The gas used for agitation can act to either, introduce new molecules to the mixture inside of the bioreactor, or remove specific metabolic molecules produced by microorganisms. Calpha Industries provides 200L-700L airlift fermenters.

Advantages

Efficient gas-liquid dispersion with low power consumption
Low shear rate (have homogenous shear and stress forces throughout the entire bioreactor.)
Improved fluidization characteristics
Excellent liquid-solid and gas-liquid mass transfer

Working Principle

Airlift bioreactor works by stirring cultured substance using gas. Air/Gas is fed through a sparger ring into the bottom of a concentric tube, which directs the circulation of both air bubbles and liquid. At the top o f the bioreactor, there is a gas separator to remove gaseous substances. Air bubbles flow upwards within the central draft tube; some of these bubbles coalesce at the top of the column and exit, while others follow the degassed liquid and circulate downward from the area outside the draft tube.